Sweet Proteins
Brazzein
Microbial Fermentation – E. coli Platform
A naturally occurring 54-amino-acid sweet protein isolated from the West African plant Pentadiplandra brazzeana. Up to 2,000× sweeter than sucrose with zero calories, exceptional heat and pH stability, and no glycemic effect.
Technical Specifications
Expression Host
Escherichia coli (Engineered Soluble Expression)
Finished Formulation
High-purity crystalline powder / Aqueous concentrate
Development Scope & Deliverables
- E. coli recombinant expression (soluble, disulfide-intact structure)
- Proof-of-Concept completed at 8L fermentation scale
- Single-step chromatography purification with high recovery
- ENK enzyme digestion & HPLC analytical method validated
- LC-MS intact mass characterization; target yield >1.8 g/L
- GRAS regulatory safety strategy & scale-up roadmap
Licensing & Tech Transfer
Request full CMC packages, analytical method validation reports, and technology transfer dossiers.
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